Maize Genetics Cooperation Newsletter vol 81 2007
Beijing, China
Chinese Academy of Sciences
RAPD analysis on the variation and mutant types induced by the special environment
of space in maize (Zea mays L.)
--Zeng, M
In our previous papers, we have observed and obtained some variation and mutant types of qualitative and quantitative traits induced by the special environment of space. For example, mutants include kernel types, multi-ear, ear length, leaf-blade, plant height, and resistance to B. maydis and E. turcicum, among others (Chinese Space Sci. Technol. 23(6):64-68, 2003; MNL 74:2-3; NNL 79:3). This paper deals with the RAPD analysis of these mutants.
Figure 1. Amplification products from operon
primers Opx1, Opx2, Opx5, Opx6, Opw1, Opw2, Opw18, Opw19, Opy15,
respectively. 1. U8112CK, 2. Mut7, 3. Me141CK, 4. Mut10, 5. Yi01-4CK, 6. Mut1, 7. XHCK, 8. XH3, 9. Zi24CK, 10. Mut4.
Experimental materials included: (1) Mut7 from U8112 and U8112CK; (2) Mut10 from Me141 and Me141CK, (3) Mut1 from Yi01-4 and Yi01-4CK, (4) XH3 from [(XH×TSPT-HZ4 Pop.)×Mut2]F2 population, (5) Mut2 from Yi01-4, and (6) Mut4 from Zi24 and Zi24 CK. Nine operons were used (Figure 1, Table 1). Molecular weight standards backgrounds are lane DL2000: 2.0Kb, 1.0Kb, 0.75Kb, 0.5Kb, 0.25Kb, 0.1Kb from top to bottom, respectively.
The results show clearly that there are changes at the molecular DNA level. Mutants (Mut7, Mut10, Mut1, Mut4) possess different amplification products compared to their CK comparison. The inbred line XH3 also possesses different amplification products.
Table 1.
Comparison of the amplification products clearly amplified from operon
primers.
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
|
|
U8112CK |
Mut7 |
Me141CK |
Mut10 |
Yi01-4CK |
Mut1 |
XHCK |
XH3 |
Zi24CK |
Mut4 |
|
|
— |
More 1.5Kb |
— |
|
— |
|
— |
More 1.5Kb |
— |
|
|
Opx1 |
|
|
|
Less 1.0Kb |
|
Less 1.1Kb |
|
|
|
Less 1.1Kb |
|
|
— |
|
— |
|
— |
|
— |
More 1.5Kb |
— |
|
|
Opx2 |
|
|
|
|
|
|
|
|
|
Less 1.1Kb |
|
|
— |
More 0.5, 1.5Kb |
— |
|
— |
|
— |
More 1.0,1.5Kb |
— |
|
|
Opx5 |
|
Less 1.1Kb |
|
|
|
|
|
|
|
Less 1.1Kb |
|
|
— |
|
— |
|
— |
|
— |
More 1.5Kb |
— |
|
|
Opx6 |
|
Less 1.5Kb |
|
|
|
|
|
|
|
Less 0.5,0.75,1.0Kb |
|
|
— |
|
— |
|
— |
More 1.0,1.5Kb |
— |
More 0.5,1.5Kb |
— |
|
|
Opw1 |
|
Less 1.0Kb |
|
|
|
|
|
|
|
Less 1.0,1.1,1.5Kb |
|
|
— |
More 1.0, 0.75Kb |
— |
More 0.5Kb |
— |
More 0.25Kb |
— |
|
— |
|
|
Opw2 |
|
|
|
|
|
|
|
Less 0.35Kb |
|
Less 1.1Kb |
|
|
— |
|
— |
More 1.1Kb |
— |
|
— |
More 1.0Kb |
— |
More 0.25Kb |
|
Opw18 |
|
|
|
|
|
|
|
|
|
Less 1.0,1.5Kb |
|
|
— |
|
— |
|
— |
|
— |
|
— |
|
|
Opw19 |
|
|
|
|
|
|
|
Less 1.1Kb |
|
Less 0.75Kb |
|
|
— |
|
— |
|
— |
More 0.4Kb |
— |
More 0.5Kb |
— |
More 0.5Kb |
|
Opy15 |
|
|
|
|
|
|
|
Less 1.75Kb |
|
|
Please Note: Notes submitted to the Maize Genetics Cooperation
Newsletter may be cited only with consent of authors.