Maize
Genetics Cooperation Newsletter 80. 2006.
In the last two newsletters (MNL 78:64-65 and MNL 79:46), we reported on the isolation and characterization of Fcu revertants. In 2004, we screened an additional population for revertants. The experiment was set up using the same genetic stocks and techniques as in 2003 (MNL 78:64-65). Out of a population of 11,118 kernels, 29 exceptional full-colored putative revertant kernels were isolated. These 29 kernels were planted this past summer and the resulting plants self-pollinated and outcrossed to the responsive r1 haplotype tester R1‑r(Venezuela559‑PI302355) in order to test for heritability and test for contamination markers. Of the 29 putative revertants, one proved to be a contaminant, lacking the y1 and wx1 markers from the Fcu parent; 26 proved not to be heritable, segregating for Fcu sectored kernels in both selfs and outcrosses; and 2 proved to be heritable germinal revertants, segregating for full colored stable kernels in both self and outcross, as well as the contamination markers. Thus, the heritable germinal reversion rate for this population is 1.8 X 10‑4. Combining these data with the 2003 and 2004 data, we obtained 3 heritable germinal revertants out of a population of 23,430 kernels, for a germinal reversion rate of 1.3 X 10‑4. The two germinal revertants isolated in the 2004 experiments have been named Fcu‑R2004‑947‑5 and Fcu‑R2004‑947‑10.
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