Maize Genetics Cooperation Newsletter 80. 2006.

 

Three point linkage data for Og*-Catlin places it on 10S

--Jackson, JD

 

       A new dominant yellow stripe stock maps to chromosome 10S near Og1.  This �phenotype only� mutation was isolated many years ago from a Dekalb hybrid.  Its phenotype is very similar to Og1.  Crosses were done with the waxy1-marked translocations: T9-10b and T8-9(043-6), and to Wx1-marked translocation T9-10(4303) to genetically determine its chromosomal location.

       The results of three-point linkage tests for Wx1, T9-10b, T9-10(4303) and Og*-Catlin are presented in Tables 1 and 2.  The linkage tests were set up as a modified backcross.  Wx and wx kernels from the backcross ears were planted in the field and the resulting plants were scored for yellow stripes and for the presence

Table 1.  Three point linkage data for Og*-Catlin-Wx1-T9-10b.

 

Testcross: wx1 N og*x [Wx1 N Og*-Catlin x wx1 T9-10b og*]

Region

Phenotype

No.

Totals

0

Wx N str

62

 

 

wx T gr

63

125

1

Wx T gr

3

 

 

wx N str

4

7

2

Wx N gr

11

 

 

wx T str

0

11

1+2

Wx T str

0

 

 

wx N gr

1

1

% recombination Wx1-T =5.6 �1.9

% recombination T-Og*=8.3 � 2.3

% recombination Wx1-*Og=13.2 � 2.8

 

Table 2.  Three point linkage data for Og*-Catlin-Wx1-T9-10(4303).

 

Testcross: wx1 N og*x [Wx1 T9-10(4303) og*x wx1 N Og*-Catlin]

Region

Phenotype

No.

Totals

0

Wx T gr

81

 

 

wx N str

76

157

1

Wx N str

3

 

 

wx T gr

2

5

2

Wx T str

0

 

 

wx N gr

5

5

1+2

Wx N gr

4

 

 

wx T str

2

6

% recombination Wx1-T =6.4 � 1.9

% recombination T-Og*=6.4 � 1.9

% recombination Wx1-*Og=12.7 � 2.5

 

of the translocation by pollen sterility.  The following linkage relationships were established:  Wx1 – 5.6 – T9-10b- 8.4 - Og*-Catlin and Wx1- 6.4 – T9-10(4303) – 6.4 - Og*-Catlin.  Crosses were undertaken with Og1 to determine if Og*-Catlin was an allele of Og1 with little success as Og1 expression was too weak to follow accurately.

       The results of two-point linkage tests for Wx1, T8-9(043-6), Inv9a and Og*-Catlin are presented in Tables 3 and 4.  The linkage tests were also set up as a modified backcross.  Wx and wx kernels from the backcross ears were planted in the field and the resulting plants were scored for yellow stripes.  Results indicate no linkage of Og*-Catlin with either chromosome 9 or chromosome 8.

 

Table 3.  Two point linkage data for  Og*-Catlin-Wx1-T8-9(043-6).

 

Testcross:   og* wx1 N x [Og*-Catlin Wx1 N x og* wx1 T8-9(043-6)]

Region

Phenotype

No.

Totals

0

Wx str

40

 

 

wx gr

48

88

1

Wx gr

45

 

 

wx str

45

90

% recombination Wx1*-Og= 50.6 � 3.7

 

Table 4.  Two point linkage data for  Og*-Catlin-Wx1-Inv9a.

 

Testcross:   og* wx1 N x [Og*-Catlin Wx1 N x og* wx1 Inv9a]

Region

Phenotype

No.

Totals

0

Wx str

16

 

 

wx gr

52

68

1

Wx gr

50

 

 

wx str

9

59

% recombination Wx1*-Og= 46.5 � 4.4

 

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The following is a correction on data previously reported in MNL74:70.  This dominant yellow stripe stock also maps to chromosome 10S near Og1.

 

Table 1.  Three point linkage data for Og*-0376-Wx1-T9-10b.

 

Testcross:   og* wx1 N x [Og*-0376 Wx1 N x og* wx1 T9-10b]

Region

Phenotype

No.

Totals

0

Wx N str

44

 

 

wx T gr

38

82

1

Wx T gr

4

 

 

wx N str.

4

8

2

Wx N gr

2

 

 

wx T str

0

2

1+2

Wx T str

0

 

 

wx N gr

1

1

% recombination Wx1-T =9.7 � 2.9

% recombination T-Og*=3.2 � 1.4

% recombination Wx1-*Og= 12.9 � 3.5 (originally reported to be 11.8 � 3.3)

 

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