Maize
Genetics Cooperation Newsletter 80. 2006.
A new dominant yellow stripe stock maps to chromosome 10S near Og1. This �phenotype only� mutation was isolated many years ago from a Dekalb hybrid. Its phenotype is very similar to Og1. Crosses were done with the waxy1-marked translocations: T9-10b and T8-9(043-6), and to Wx1-marked translocation T9-10(4303) to genetically determine its chromosomal location.
The
results of three-point linkage tests for Wx1,
T9-10b, T9-10(4303) and Og*-Catlin are presented in Tables 1 and 2. The linkage tests were set up as a
modified backcross. Wx and wx
kernels from the backcross ears were planted in the field and the resulting
plants were scored for yellow stripes and for the presence
Table 1. Three point linkage data for Og*-Catlin-Wx1-T9-10b.
Testcross: wx1 N og*x [Wx1 N Og*-Catlin x wx1 T9-10b og*]
|
Region |
Phenotype |
No. |
Totals |
|
0 |
Wx N str |
62 |
|
|
|
wx T gr |
63 |
125 |
|
1 |
Wx T gr |
3 |
|
|
|
wx N str |
4 |
7 |
|
2 |
Wx N gr |
11 |
|
|
|
wx T str |
0 |
11 |
|
1+2 |
Wx T str |
0 |
|
|
|
wx N gr |
1 |
1 |
% recombination Wx1-T =5.6 �1.9
% recombination T-Og*=8.3 � 2.3
% recombination Wx1-*Og=13.2 � 2.8
Table
2. Three point linkage data for Og*-Catlin-Wx1-T9-10(4303).
Testcross: wx1 N og*x [Wx1 T9-10(4303) og*x wx1 N Og*-Catlin]
|
Region |
Phenotype |
No. |
Totals |
|
0 |
Wx T gr |
81 |
|
|
|
wx N str |
76 |
157 |
|
1 |
Wx N str |
3 |
|
|
|
wx T gr |
2 |
5 |
|
2 |
Wx T str |
0 |
|
|
|
wx N gr |
5 |
5 |
|
1+2 |
Wx N gr |
4 |
|
|
|
wx T str |
2 |
6 |
% recombination Wx1-T =6.4 �
1.9
% recombination T-Og*=6.4 � 1.9
% recombination Wx1-*Og=12.7 � 2.5
of the translocation by pollen sterility. The
following linkage relationships were established: Wx1 –
5.6 – T9-10b- 8.4 - Og*-Catlin
and Wx1- 6.4 – T9-10(4303)
– 6.4 - Og*-Catlin. Crosses were undertaken with Og1 to determine if Og*-Catlin was an allele of Og1 with little success as Og1 expression was too weak to follow accurately.
The results of two-point linkage tests for Wx1, T8-9(043-6), Inv9a and Og*-Catlin are presented in Tables 3 and 4. The linkage tests were also set up as a modified backcross. Wx and wx kernels from the backcross ears were planted in the field and the resulting plants were scored for yellow stripes. Results indicate no linkage of Og*-Catlin with either chromosome 9 or chromosome 8.
Table
3. Two point linkage data for Og*-Catlin-Wx1-T8-9(043-6).
Testcross: og* wx1 N x [Og*-Catlin Wx1 N x og* wx1
T8-9(043-6)]
|
Region |
Phenotype |
No. |
Totals |
|
0 |
Wx str |
40 |
|
|
|
wx gr |
48 |
88 |
|
1 |
Wx gr |
45 |
|
|
|
wx str |
45 |
90 |
% recombination Wx1*-Og= 50.6 � 3.7
Table
4. Two point linkage data for Og*-Catlin-Wx1-Inv9a.
Testcross: og* wx1 N x [Og*-Catlin Wx1 N x og* wx1
Inv9a]
|
Region |
Phenotype |
No. |
Totals |
|
0 |
Wx str |
16 |
|
|
|
wx gr |
52 |
68 |
|
1 |
Wx gr |
50 |
|
|
|
wx str |
9 |
59 |
% recombination Wx1*-Og= 46.5 � 4.4
-------------------------------------------------------------------------------------------------------------------------------
The following is a correction on data previously reported in
MNL74:70. This dominant yellow stripe stock also maps to
chromosome 10S near Og1.
Table
1. Three point linkage data for Og*-0376-Wx1-T9-10b.
Testcross: og* wx1 N x [Og*-0376 Wx1 N x og* wx1 T9-10b]
|
Region |
Phenotype |
No. |
Totals |
|
0 |
Wx N str |
44 |
|
|
|
wx T gr |
38 |
82 |
|
1 |
Wx T gr |
4 |
|
|
|
wx N str. |
4 |
8 |
|
2 |
Wx N gr |
2 |
|
|
|
wx T str |
0 |
2 |
|
1+2 |
Wx T str |
0 |
|
|
|
wx N gr |
1 |
1 |
% recombination Wx1-T =9.7 �
2.9
% recombination T-Og*=3.2 � 1.4
% recombination Wx1-*Og= 12.9 � 3.5 (originally
reported to be 11.8 � 3.3)
_________________________________________________
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note:
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