Maize
Genetics Cooperation Newsletter 80. 2006.
In the collection of A-A translocation stocks maintained at MGCSC is a series of waxy1-linked translocations that are used for mapping unplaced mutants. Also, new wx1-linked translocations are being introduced into this series and are in a conversion program to convert each translocation to the inbred backgrounds M14 and W23. These inbred conversions are then crossed together to produce vigorous hybrids to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and can now supply good sources proven by linkage tests to include the correct translocated chromosomes. Additional pedigree information on bad sources is available should anyone want to check on sources supplied to them previously by the Stock Center.
Previously we reported the linkage results for some of these stocks (MNL72:81-82; MNL73:88-89; MNL74:67-69; MNL75:68-71; MNL76:65-67; MNL77:79; MNL78:65-66; MNL79:47).
Table 1 contains linkage results for an additional wx1-linked translocation stock we have completed testing. This is a stock received from Susan Gabay-Laughnan. She originally obtained it from our 2084B Stock.
Table
1. wx1 T8-9(043-6) (8L.17;
9S.34)
A) The new source showed linkage of wx1 with v16:
2 point linkage data for v16-wx1 T8-9(043-6)
Testcross: [V16 wx1 T8-9(043-6) x v16 Wx1 N] x v16 wx1
N
source:SGL2003-289-1
|
Region |
Phenotype |
No. |
Totals |
|
0 |
v Wx |
1298 |
|
|
|
+ wx |
1856 |
3154 |
|
1 |
+ Wx |
346 |
|
|
|
v wx |
345 |
691 |
% recombination v16-wx1=18.0 � 0.6
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Following is a correction for Table 6 in Jackson, J and Stinard, P. 1998. MNL72:81-82. The % recombination reported was between bf2 and Wx1 not r1 and Wx1 as stated.
Table
6. wx1 T9-10b (9S.13; 10S.40)
A) The M14 sources showed linkage of wx1 with bf2.
2 point linkage data for bf2-wx1 T9-10b
Testcross: [Bf2 wx1 T9-10b
x bf2 Wx1 N] x bf2 wx1 N
source:82-116-1^M14
|
Region |
Phenotype |
No. |
Totals |
|
0 |
bf Wx |
334 |
|
|
|
+ wx |
312 |
646 |
|
1 |
+ Wx |
46 |
|
|
|
bf wx |
19 |
65 |
% recombination bf2-Wx1=9.1 �
1.1
B) The W23 sources showed linkage of wx1 with bf2.
2 point linkage data for bf2-wx1 T9-10b
Testcross: [Bf2 wx1 T9-10b
x bf2 Wx1 N] x bf2 wx1 N
source:82-117-1^W23
|
Region |
Phenotype |
No. |
Totals |
|
0 |
bf Wx |
812 |
|
|
|
+ wx |
892 |
1704 |
|
1 |
+ Wx |
40 |
|
|
|
bf wx |
39 |
79 |
% recombination bf2-Wx1=4.4
� 0.5
_________________________________________________
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note:
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with consent of the authors.
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