Maize
Genetics Cooperation Newsletter 80. 2006.
Maize Genetics Cooperation Stock Center
Approximately 1 acre each year is devoted to the propagation of the large collection of A-A translocation stocks. In this collection is a series of Waxy1-linked translocations that are used for mapping unplaced mutants. Each translocation is maintained in separate M14 and W23 inbred backgrounds which are crossed together to produce vigorous hybrids to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and can now supply good sources proven by linkage tests to include the correct translocated chromosomes.
Previously we reported the linkage results for some of these stocks (MNL72:79-81; MNL73:86-88; MNL74:67; MNL75:67; MNL76:67-68; MNL77:80; MNL79:48). Below is a summary of additional translocation stocks we have completed testing.
Table
1. Wx1 T1-9c
(1S.48; 9L.22)
A) The F1 source showed linkage of wx1 with P1-ww:
2 point linkage data for P1-ww-Wx1 T1-9c
Testcross: [P1-wr wx1 N x P1-ww Wx1 T1-9c] x P1-ww wx1 N wx1
source:87-948 x 951^F1
|
Region |
Phenotype |
No. |
Totals |
|
0 |
P1-ww Wx |
186 |
|
|
|
P1-wr wx |
184 |
370 |
|
1 |
P1-wr Wx |
20 |
|
|
|
P1-ww wx |
12 |
32 |
% recombination P1-ww-Wx1=8.0 �
1.4
-------------------------------------------------------------------------------------------------------------------------------
Table
2. Wx1 T5-9a (5L.69; 9S.17)
The Wx1-marked sources for T5-9a showed
no linkage with v2. New crossovers
were recovered from the wx1-marked
sources and checked for linkage with v2
A) The 4 new M14 crossovers showed no linkage
with v2 and were discarded.
B) One of the 2 new W23 crossovers showed
linkage with v2 and is
being converted to M14 to produce vigorous F1s.
2 point linkage data
for v2-Wx1 T5-9a
Testcross: [v2 wx1 N x V2 Wx1 T5-9a] x v2 wx1 N
source: 2002P-388-10 from 2001-1005-6c/o^W23
|
Region |
Phenotype |
No. |
Totals |
|
0 |
+ Wx |
1504 |
|
|
|
v wx |
1542 |
3046 |
|
1 |
v Wx |
47 |
|
|
|
+ wx |
58 |
105 |
% recombination v2-Wx1= 3.3
� 0.3
Subsequent
generations of this new crossover were checked to confirm linkage with v2.
source: 2003-1067-1 from 2002P-388-10 from 2001-1005-6c/o^W23
|
Region |
Phenotype |
No. |
Totals |
|
0 |
+ Wx |
1466 |
|
|
|
v wx |
1331 |
2797 |
|
1 |
v Wx |
52 |
|
|
|
+ wx |
54 |
106 |
% recombination v2-Wx1= 3.7
� 0.3
source: 2003-1067-2 from 2002P-388-10 from 2001-1005-6c/o^W23
|
Region |
Phenotype |
No. |
Totals |
|
0 |
+ Wx |
1391 |
|
|
|
v wx |
1354 |
2745 |
|
1 |
v Wx |
51 |
|
|
|
+ wx |
48 |
99 |
% recombination v2-Wx1= 3.5 �
0.3
source: 2003-1067-4 from 2002P-388-10 from 2001-1005-6c/o^W23
|
Region |
Phenotype |
No. |
Totals |
|
0 |
+ Wx |
1213 |
|
|
|
v wx |
1264 |
2477 |
|
1 |
v Wx |
79 |
|
|
|
+ wx |
47 |
126 |
% recombination v2-Wx1= 4.8 �
0.4
source: 2003-1067-5 from 2002P-388-10 from 2001-1005-6c/o^W23
|
Region |
Phenotype |
No. |
Totals |
|
0 |
+ Wx |
1435 |
|
|
|
v wx |
1512 |
2947 |
|
1 |
v Wx |
75 |
|
|
|
+ wx |
55 |
130 |
% recombination v2-Wx1= 4.2 �
0.4
source: 2003-1067-7 from 2002P-388-10 from 2001-1005-6c/o^W23
|
Region |
Phenotype |
No. |
Totals |
|
0 |
+ Wx |
1526 |
|
|
|
v wx |
1656 |
3182 |
|
1 |
v Wx |
78 |
|
|
|
+ wx |
97 |
175 |
% recombination v2-Wx1=5.2 � 0.4
source: 2003-1067-10 from 2002P-388-10 from 2001-1005-6c/o^W23
|
Region |
Phenotype |
No. |
Totals |
|
0 |
+ Wx |
829 |
|
|
|
v wx |
848 |
1677 |
|
1 |
v Wx |
39 |
|
|
|
+ wx |
47 |
86 |
_________________________________________________
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note:
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with consent of the authors.
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