MUNCIE, INDIANA
Ball State University1
WOODWARD, OKLAHOMA
USDA-ARS-SPRRS2 (posthumously, 10/02)
Tripsacum SR5 Genetic Map - a new generation
--Blakey, CA1, Dewald, CL2
The establishment of a single-site location for the new molecular genetic
mapping work and mapping population of Tripscaum dactyloides was
undertaken at Ball State University. This map will build on information
gained in the construction of a previous map in Tripsacum dactyloides,
which had been limited by a combination of sample size and field-nursery
contamination. All plants are being maintained at Ball State in a non-flowering,
vegetative growth stage to avoid these problems and allow for a continuing
supply of F2 tissue for research. Currently, the only source of the paternal
plant material of this F2 is housed at Ball State University. The primary
focus of this project will be to derive a cross-genera marker genetic map
of the warm season perennial forage grass Tripsacum dactyloides, a
relative of modern maize, as a genetic resource for crop improvement in
the Gramineae. The map will combine data from three sets of markers: 1)
the TDA markers (derived from genomic DNA of Tripsacum dactyloides)
developed by the PI while at the Univ. of Missouri; 2) the UMC RFLP maize
bin markers; and 3) the Cornell Anchor marker set, which includes markers
from several genera of the Gramineae. A broad-based marker map in Tripsacum,
as a member of the Gramineae, utilizing a combination of species-derived
and cross-specific markers will provide an enhanced basis for trait specific
investigations, such as apomixis and seed shattering. The mapping population
will eventually consist of 120 - 150 F2 individuals from the parental cross
WW1673 x WW1760, and is known to segregate for the distinct recessive floral
genotype gynomonoecious sex form-1 (a known homolog to the floral
gene ts2, in Zea mays, Li et al. PNAS 1997). The mapping
individuals are a subset of a population currently under evaluation by
the USDA for quantitative trait analyses of seed shattering and forage
quality.
Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors.
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