BERKELEY, CALIFORNIA
University of California-Berkeley
Molecular characterization of mutations resulting in a liguleless1
phenotype
--Braun, DM, Freeling, M
In our studies on liguleless1 (lg1) we have characterized many independent
mutations with a liguleless phenotype. These mutants were obtained from
the Maize Coop Genetic Stock Center, sent to us by other collaborators,
or identified in the Freeling lab. All were determined to be alleles of
lg1
by complementation testing the new liguleless mutants to the
lg1-Reference
allele. All of the F1 progeny showed a mutant phenotype. The F1 were self-fertilized
and the F2 displayed a 100% mutant phenotype indicating that the new mutant
being tested was allelic to lg1. All of the alleles have a phenotype
indistinguishable from the reference allele with the exception of lg1-N2375
which has a much weaker phenotype. The nature of the lesion in the lg1
gene was determined for many of the alleles (Table 1). To characterize
each mutation, a combination of Southern blot analysis, PCR amplification
and DNA sequence analysis of the coding region of lg1 was performed.
Note that for three alleles we were unable to find any changes in the coding
region; these alleles likely represent cis regulatory mutations.
Table 1. Molecular alterations in various lg1 alleles.
|
Allele
|
Alteration
|
|
lg1-Reference
|
Deletion of entire gene
|
|
lg1-64-4
|
Deletion of entire gene
|
|
lg1-6198
|
Deletion of entire gene
|
|
lg1-K16
|
Deletion of entire gene
|
|
lg1-Ds
|
Deletion of entire gene
|
|
lg1-PI200299
|
268 bp deletion of promoter, transcription & translation start
sites
|
|
lg1-m1
|
Ac insertion in intron1
|
|
lg1-MTM
|
Mu insertion in 5� end
|
|
lg1-PI262493
|
Tourist insertion in exon2
|
|
lg1-TaiTaiTaSarga
|
4 bp insertion in exon2
|
|
lg1-ZCxGRB
|
Insertion in 5� end
|
|
lg1-128
|
Insertion in 5� end
|
|
lg1-340
|
Insertion in 5� end
|
|
lg1-656
|
Insertion in 5� end
|
|
lg1-N2375
|
V173M mutation in DNA binding domain
|
|
lg1-m2
|
unknown
|
|
lg1-ems
|
unknown
|
|
lg1-56-30375
|
unknown
|
Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors.
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