Small transposable elements isolated
from transcripts of the intensifier alleles in and In-D
--Pusch, I, Herrmann, M, Hoogvliet,
O, Prause, A, Scheffler, B*, Lorbiecke, R, Wienand, U
*USDA, ARS, Natural Products Utilization
Research Unit
Sequence analysis of clones of the recessive
intensifier allele in and the dominant allele Intensifier-Dilute
(In-D) revealed the presence of small transposable elements in the
genomic and cDNAs of both alleles. In allele In-D, a 122 bp transposable
element called BEB is present in exon 6 of this allele. BEB
is also found in transcripts of In-D and leads to non-functional
proteins. The BEB element has also been found in a seedling cDNA
isolated from a W22 color converted line (Line C). The insert is absent
in an EST clone isolated from a tassel cDNA library of line Oh43. A 315
bp transposable element called ROH was found in the misspliced transcripts
of the recessive allele in. The ROH element is integrated at the
3ยด end of the second intron of in and was also found in truncated
transcripts of in. The termination of in mRNAs in the second
intron might be due to a poly-adenylation site in the ROH element.
So far no other sequence homologies to the ROH and BEB elements
have been found in the reported maize genome.
Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors.
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