Small transposable elements isolated from transcripts of the intensifier alleles in and In-D --Pusch, I, Herrmann, M, Hoogvliet, O, Prause, A, Scheffler, B*, Lorbiecke, R, Wienand, U
*USDA, ARS, Natural Products Utilization Research Unit
Sequence analysis of clones of the recessive intensifier allele in and the dominant allele Intensifier-Dilute (In-D) revealed the presence of small transposable elements in the genomic and cDNAs of both alleles. In allele In-D, a 122 bp transposable element called BEB is present in exon 6 of this allele. BEB is also found in transcripts of In-D and leads to non-functional proteins. The BEB element has also been found in a seedling cDNA isolated from a W22 color converted line (Line C). The insert is absent in an EST clone isolated from a tassel cDNA library of line Oh43. A 315 bp transposable element called ROH was found in the misspliced transcripts of the recessive allele in. The ROH element is integrated at the 3ยด end of the second intron of in and was also found in truncated transcripts of in. The termination of in mRNAs in the second intron might be due to a poly-adenylation site in the ROH element. So far no other sequence homologies to the ROH and BEB elements have been found in the reported maize genome.
 
 
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