COLUMBIA, MISSOURI
University of Missouri
URBANA, ILLINOIS
University of Illinois and USDA/ARS
Genetic mapping of zebra3
--Rugen, M., Stinard, PS, Cone, KC
The recessive zebra3 (zb3)
phenotype is apparent as a distinct light-green crossbanding on seedling
and adult leaves of the maize plant. In an earlier study (MNL 73:23), we
showed that previous reports that zb3 maps on 5L were incorrect.
To determine the correct map location, zb3 was crossed by a series
of TB stocks; the results indicated that zb3 is on the short arm
of chromosome 1. For more specific localization, we used molecular segregation
analysis. We generated a segregating population by crossing zb3
(Coop stock 519G) to a W23/L317 hybrid and then self-pollinating the F1
to generate an F2. F2 seed were planted and the phenotypes scored; 27 of
100 plants had the zb3 phenotype. DNA was isolated from the zb3
plants, digested with restriction enzymes, blotted and probed with markers
from chromosome 1S. The following data were obtained:
| probe |
# chromosomes tested
|
# crossovers
|
recombination frequency (%)
|
| tub1 |
54
|
0
|
0
|
| asg31 |
54
|
3
|
5.6
|
| umc157 |
54
|
2
|
3.7
|
| asg45 |
54
|
8
|
14.8
|
We conclude that zb3 is tightly
linked to tub1.
Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors.
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