Approximately 1 acre each year is devoted to the propagation of the large collection of A-A translocation stocks. In this collection is a series of Waxy1-linked translocations that are used for mapping unplaced mutants. Each translocation is maintained in separate M14 and W23 inbred backgrounds which are crossed together to produce vigorous F1's to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and we can now supply good sources proven by linkage tests to include the correct translocated chromosomes. Below is a summary of additional translocation stocks we have completed testing.
Table 1. Wx1 T2-9b F1 (2S.18; 9L.22)
A) The F1 source showed linkage of wx1 with v4:
2 point linkage data for v4-Wx1 T2-9b
Testcross: [V4 Wx1 T2-9b x v4 wx1 N] x v4 wx1
N
source:87-966 x 964 F1 of ^M14 x ^W23
Region | Phenotype | No. | Totals |
0 | + Wx | 1629 | |
v wx | 1342 | 2971 | |
1 | v Wx | 205 | |
+ wx | 175 | 380 |
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Table 2. Wx1 T5-9d (5L.14; 9L.10)
A) The M14 sources showed linkage of wx1 with gl8:
2 point linkage data for gl8-Wx1 T5-9d
Testcross: [Gl8 Wx1 T5-9d x gl8 wx1 N] x gl8 wx1
N
source:93W-1449 ^M14
Region | Phenotype | No. | Totals |
0 | + Wx | 58 | |
gl wx | 42 | 100 | |
1 | gl Wx | 15 | |
+ wx | 24 | 39 |
source:94-1867-1 ^M14
Region | Phenotype | No. | Totals |
0 | + Wx | 497 | |
gl wx | 492 | 989 | |
1 | gl Wx | 129 | |
+ wx | 131 | 260 |
source:94-1869-1 ^M14
Region | Phenotype | No. | Totals |
0 | + Wx | 1332 | |
gl wx | 1282 | 2614 | |
1 | gl Wx | 331 | |
+ wx | 272 | 603 |
B) The W23 sources showed no linkage of wx1 with gl8.
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Table 3. Wx1 T7-9(4363) (7ctr.; 9ctr.)
A) Some M14 sources showed linkage of wx1 with gl1:
2 point linkage data for gl1-Wx1 T7-9(4363)
Testcross: [gl1 Wx1 T7-9(4363) x Gl1 wx1 N] x gl1
wx1 N
source:94-1874 ^M14
Region | Phenotype | No. | Totals |
0 | gl Wx | 98 | |
+ wx | 101 | 199 | |
1 | + Wx | 13 | |
gl wx | 8 | 21 |
source:95-889-2 ^M14
Region | Phenotype | No. | Totals |
0 | gl Wx | 931 | |
+ wx | 942 | 1873 | |
1 | + Wx | 49 | |
gl wx | 35 | 84 |
source:76-2062-1 ^M14
Region | Phenotype | No. | Totals |
0 | gl Wx | 428 | |
+ wx | 434 | 862 | |
1 | + Wx | 36 | |
gl wx | 12 | 48 |
B) Some W23 sources showed linkage of wx1 with gl1:
2 point linkage data for gl1-Wx1 T7-9(4363)
Testcross: [gl1 Wx1 T7-9(4363) x Gl1 wx1 N] x gl1
wx1 N
source:87-1029 ^W23
Region | Phenotype | No. | Totals |
0 | gl Wx | 663 | |
+ wx | 731 | 1394 | |
1 | + Wx | 19 | |
gl wx | 12 | 31 |
source:94-1876 ^W23
Region | Phenotype | No. | Totals |
0 | gl Wx | 612 | |
+ wx | 622 | 1234 | |
1 | + Wx | 38 | |
gl wx | 18 | 56 |
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Table 4. Wx1 T9-10(8630) (9S.28; 10L.37)
A) Unconverted source showed linkage of wx1 with r1:
2 point linkage data for r1-Wx1 T9-10(8630)
Testcross: [r1 Wx1 T9-10(8630) x R1 wx1
N] x r1 wx1 N
source:72-3342-2
Region | Phenotype | No. | Totals |
0 | r Wx | 935 | |
+ wx | 850 | 1785 | |
1 | r wx | 120 | |
+ Wx | 174 | 294 |
B) Additional W23 sources showed linkage of wx1 with r1:
2 point linkage data for r1-Wx1 T9-10(8630)
Testcross: [r1 Wx1 T9-10(8630) x R1 wx1
N] x r1 wx1 N
source:87-1077 ^W23
Region | Phenotype | No. | Totals |
0 | r Wx | 1445 | |
+ wx | 1085 | 2530 | |
1 | r wx | 172 | |
+ Wx | 250 | 422 |
source:93W-1459-5 ^W23
Region | Phenotype | No. | Totals |
0 | r Wx | 1052 | |
+ wx | 736 | 1788 | |
1 | r wx | 117 | |
+ Wx | 192 | 309 |
source:93W-1459-6 ^W23
Region | Phenotype | No. | Totals |
0 | r Wx | 1213 | |
+ wx | 910 | 2123 | |
1 | r wx | 136 | |
+ Wx | 248 | 384 |
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