Approximately 1 acre each year is devoted to the propagation of the large collection of A-A translocation stocks. In this collection is a series of Waxy1-linked translocations that are used for mapping unplaced mutants. Each translocation is maintained in separate M14 and W23 inbred backgrounds which are crossed together to produce vigorous F1's to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and we can now supply good sources proven by linkage tests to include the correct translocated chromosomes. Additional translocation stocks will be tested as time allows. Below is a summary of those we have completed testing.
Table 1. Wx1 T3 -9(8562) (3L.65; 9L.22).
A) The M14 sources showed linkage of wx1 with a1.
B) The W23 sources showed linkage of wx1 with a1.
A) 2 point linkage data for a1-Wx1 T3-9(8562)
Testcross: [A1 Wx1 T3-9(8562) x a1 wx1
N] x a1 wx1 N
source:93W-1423-1 ^M14
Region | Phenotype | No. | Totals |
0 | + Wx | 675 | |
a wx | 656 | 1331 | |
1 | a Wx | 174 | |
+ wx | 174 | 348 |
B) 2 point linkage data for a1-Wx1 T3-9(8562)
Testcross: [A1 Wx1 T3-9(8562) x a1 wx1
N] x a1 wx1 N
source:93W-1425-2 ^W23
Region | Phenotype | No. | Totals |
0 | + Wx | 748 | |
a wx | 607 | 1355 | |
1 | a Wx | 193 | |
+ wx | 201 | 394 |
Table 2. Wx1 T4 -9g (4S.27; 9L.27).
A) Some F1's showed linkage of wx1 with su1.
B) Some of the M14 sources showed linkage of wx1 with su1:
C) Some of the W23 sources showed linkage of wx1 with su1:
A) 2 point linkage data for su1-Wx1 T4-9g
Testcross: [Su1 Wx1 T4-9g x su1 wx1 N]
x
su1 wx1 N
source:87-888 x 990-8 F1 of ^M14 x ^W23
Region | Phenotype | No. | Totals |
0 | + Wx | 1725 | |
su wx | 1074 | 2799 | |
1 | su Wx | 43 | |
+ wx | 92 | 135 |
source:87-889 x 990-8 F1 of ^M14 x ^W23
Region | Phenotype | No. | Totals |
0 | + Wx | 2339 | |
su wx | 1807 | 4146 | |
1 | su Wx | 100 | |
+ wx | 74 | 174 |
B) 2 point linkage data for su1-Wx1 T4-9g
Testcross: [Su1 Wx1 T4-9g x su1 wx1 N]
x
su1 wx1 N
source:82-215-5^M14
Region | Phenotype | No. | Totals |
0 | + Wx | 1544 | |
su wx | 1212 | 2756 | |
1 | su Wx | 30 | |
+ wx | 48 | 78 |
source:84-15-2^M14
Region | Phenotype | No. | Totals |
0 | + Wx | 290 | |
su wx | 234 | 524 | |
1 | su Wx | 6 | |
+ wx | 5 | 11 |
source:84-15-4^M14
Region | Phenotype | No. | Totals |
0 | + Wx | 1123 | |
su wx | 896 | 2019 | |
1 | su Wx | 35 | |
+ wx | 56 | 91 |
source:79-105-5^M14
Region | Phenotype | No. | Totals |
0 | + Wx | 870 | |
su wx | 718 | 1588 | |
1 | su Wx | 28 | |
+ wx | 28 | 56 |
C) 2 point linkage data for su1-Wx1 T4-9g
Testcross: [Su1 Wx1 T4-9g x su1 wx1 N]
x
su1 wx1 N
source:93W-1443-3^W23
Region | Phenotype | No. | Totals |
0 | + Wx | 275 | |
su wx | 217 | 492 | |
1 | su Wx | 10 | |
+ wx | 15 | 25 |
Table 3. Wx1 T5-9c (5S.07; 9L.10).
A) The M14 source showed linkage of wx1 with a2.
B) The W23 source showed no linkage of wx1 with a2.
A) 2 point linkage data for a2-Wx1 T5-9c
Testcross: [A2 Wx1 T5-9c x a2 wx1 N] x
a2
wx1 N
source:82-142-3 ^M14
Region | Phenotype | No. | Totals |
0 | + Wx | 1545 | |
a wx | 1359 | 2904 | |
1 | a Wx | 65 | |
+ wx | 66 | 131 |
Table 4. Wx1 T5-9(4817) (5L.06; 9S.07).
A) The M14 source showed linkage of wx1 with a2:
B) Some W23 sources showed linkage of wx1 with a2.
A) 2 point linkage data for a2-Wx1 T5-9(4817)
Testcross: [A2 Wx1 T5-9(4817) x a2 wx1
N] x a2 wx1 N
source:93W-1445-3 ^M14
Region | Phenotype | No. | Totals |
0 | + Wx | 343 | |
a wx | 406 | 749 | |
1 | a Wx | 15 | |
+ wx | 19 | 34 |
source:93W-1445-3 ^M14
Region | Phenotype | No. | Totals |
0 | + Wx | 912 | |
a wx | 886 | 1798 | |
1 | a Wx | 65 | |
+ wx | 47 | 112 |
B) 2 point linkage data for a2-Wx1 T5-9(4817)
Testcross: [A2 Wx1 T5-9(4817) x a2 wx1
N] x a2 wx1 N
source:84-07-8 ^W23
Region | Phenotype | No. | Totals |
0 | + Wx | 1796 | |
a wx | 1696 | 3492 | |
1 | a Wx | 96 | |
+ wx | 97 | 193 |
source:85-019-1 ^W23
Region | Phenotype | No. | Totals |
0 | + Wx | 487 | |
a wx | 489 | 976 | |
1 | a Wx | 11 | |
+ wx | 16 | 27 |
Table 5. Wx1 T9-10(8630) (9S.28; 10L.37).
A) All of the M14 sources showed no linkage of wx1 with either
r1
or g1.
B) Some of the W23 sources showed linkage of wx1 with r1
& g1:
B) 2 point linkage data for r1-Wx1 T9-10(8630)
Testcross: [r1 Wx1 T9-10(8630) x R1 wx1
N] x r1 wx1 N
source:93W-1459-5^W23
Region | Phenotype | No. | Totals |
0 | r Wx | 1187 | |
+ wx | 992 | 2179 | |
1 | r wx | 157 | |
+ Wx | 377 | 534 |
B) 2 point linkage data for g1-Wx1 T9-10(8630)
Testcross: [G1 Wx1 T9-10(8630) x g1 wx1
N ] x g1 wx1 N
source:93W-1459-5^W23
Region | Phenotype | No. | Totals |
0 | + Wx | 761 | |
g wx | 755 | 1516 | |
1 | g Wx | 47 | |
+ wx | 25 | 72 |
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