A gene conditioning high oleic maize oil, olc1
--Allen Wright
Maize (Zea mays L.) oil with higher levels of oleic acid relative to linoleic acid would have greater stability. Thus high oleic acid oil is a desirable breeding objective. From EMS-treated B73 (paraffin oil pollen treatment) a mutation has been recovered that increases oleic acid concentration of the seed oil. This mutation (designated Olc1 ) has a partially dominant effect . The oleic acid concentrations of non-mutant B73, heterozygous Olc1 and homozygous Olc1 were approximately 27, 35 and 52%, respectively (Table 1). B-A translocations (Table 2) indicate that the Olc1 gene is located on the long arm of chromosome 1.
Table 1. Chi square analysis of olc1/Olc1 F2 and olc1/Olc1
x olc1/olc1 segregations
| olc1/Olc1 F2 | Obs. | Exp. | |
| Phenotype | (x) | (m) | (x-m2)/m |
| Low oleic: olc1/olc1
(Range: 24.8 - 30.1% oleic) |
27 | 25 | 0.16 |
| Intermediate oleic:olc1/Olc1
(Range: 32.4 - 38.1% oleic) |
52 | 50 | 0.08 |
| High oleic: Olc1/Olc1
(Range: 50.3 - 55.2% oleic) |
21 | 25 | 0.64 |
| Chi square=0.88 P=64.54% | |||
| olc1/Olc1 X olc1/olc1 | Obs. | Exp. | |
| Phenotype | (x) | (m) | (x-m2)/m |
| Low oleic: olc1/olc1
(Range: 25.5 - 30.1%) |
36 | 35 | 0.0286 |
| Intermediate oleic: Olc1/olc1
(Range: 33.0 - 38.1%) |
34 | 35 | 0.0286 |
| Chi square=0.0572 P=81.27% |
Table 2. Maximum oleic acid content of B-A translocation crosses to
mutant (Olc1) and non-mutant B73 (maximum value of at least 10 individual
kernel determinations).
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