Effect of media on callusing and rhizogenesis from cultured root explants of genotype TUXP237-2
--Ashok Kumar, Harsh Kumar, S. K. T. Nasar and M. Kumar

The success of tissue culture experiments depends on selection of suitable media combinations. Thus, in order to select suitable media for callusing as well as for rhizogenesis, root explants from in vitro germinated seedlings of the genotype TUXP237-2 were inoculated in 15 different MS media combinations supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), indole butyric acid (IBA), indole 3-acetic acid (IAA) and kinetin (KN), either singly or in combinations of two. The responses were recorded after 45-50 days of culture. The best response with 2,4-D and IBA was at 5 mgl-1 and 2.5 mgl-1 respectively, with 2,4-D favouring more callusing and IBA favouring more rooting. Among the media with 2,4-D and IAA, the maximum response was observed at 2,4-D 5 mgl-1 and IAA 2 mgl-1. Similarly, the best response with 2,4-D and IBA was with 5 mgl-1 and 2.5 mgl-1. When 2,4-D was used with KN, the response was moderated and for this combination the maximum response was found when 2,4-D was used at 3 mgl-1 and KN at 2 mgl-1 (Table 1). Normally the medium favouring callusing also favoured rhizogenesis.

Table 1. Callusing and rhizogenesis from cultured root explants on different media.
 
Callusing
Rhizogenesis
SI no.
Medium MS plus
% cultures
Growth
% cultures
No.
1 2,4-D 0.5 mgl-1
55.55
++
27.77
2-4
2 2,4-D 2.5mgl-1 61.11 ++ 44.44 4-6
3 2,4-D 5.0 mgl-1 72.22 +++ 55.55 4-8
4 IBA 0.5 mgl-1 55.55 ++ 66.66 8-10
5 IBA 2.5 mgl-1 66.66 +++ 94.44 15-20
6 IBA 5.0 mgl-1 50.00 ++ 72.22 8-12
7 2,4-D 0.5 mgl-1 + IAA 0.5 mgl-1 44.44 +++ 44.44 2-6
8 2,4-D 2.0 mgl-1 + IAA 5.0 mgl-1 66.66 +++ 55.55 4-7
9 2,4-D 5.0 mgl-1 + IAA 2.0 mgl-1 88.88 ++++ 66.66 5-10
10 2,4-D 0.5 mgl-1 + IBA 0.5 mgl-1 55.55 ++ 55.55 2-6
11 2,4-D 2.5 mgl-1 + IBA 0.5 mgl-1 55.55 +++ 61.11 6-8
12 2,4-D 5.0 mgl-1 + IBA 2.5 mgl-1 77.77 ++++ 72.22 6-12
13 2,4-D 0.5 mgl-1 + KN 0.5 mgl-1 27.77 + 33.33 2-4
14 2,4-D 3.0 mgl-1 + KN 2.0 mgl-1 33.33 ++ 38.88 2-6
15 2,4-D 5.0mgl-1+KN 5.0 mgl-1 22.22 + 27.77 1-3
Callusing - + = low; ++ = moderate; +++ = high; ++++ = very high 

Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors

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