Transposon tagging the su1 locus
--M. J. Scanlon, M. G. James, P. S. Stinard, D. S. Robertson and A.
M. Myers
We are interested in transposon tagging and cloning genes involved in
endosperm development. Included among the kernel mutants grown in our 1991
nursery were plants from seven putative Mutator-tagged su1 allelic
families identified by D. S. Robertson, DS. The laboratory designations
of this allelic series are given below:
su1-Mu*2412 su1-Mu*7110
su1-Mu*8064 su1-Mu*5167
su1-Mu*3217 su1-Mu*4582
su1-Mu*3162
Southern blot analysis of genomic DNA derived from su1-Mu*2412 seedlings
has revealed a Mu1-tagged 2.2Kb HindIII fragment that cosegregates
with the su1 phenotype. This family also cosegregates a 4.0Kb Mu1
homologous EcoRI fragment. The 2.2Kb HindIII genomic fragment
has recently been cloned into phage lambda NM1149 and purified. Efforts
are now underway to isolate genomic DNA flanking the Mu1 insertion
of this clone. Flanking DNA will then be used as a molecular probe in Northern
analyses of Su1 transcript, cloning of the wildtype cDNA, and RFLP
mapping of putative QTL's for kernel size.
Please Note: Notes submitted to the Maize Genetics Cooperation
Newsletter may be cited only with consent of the authors
Return to the MNL 66 On-Line Index
Return to the Maize Newsletter Index
Return to the MaizeGDB Homepage