Brookhaven National Laboratory
RFLP mapping of mutants
--Eileen C. Matz, Frances A. Burr and Benjamin Burr
We are interested in correlating the RFLP map based on recombinant inbred
families T232xCM37 and Tx303xCO159, with the traditional linkage map in
regions of the genome where the RFLP map is not marked with common loci.
We also have attempted to map two loci that had been mapped only to chromosome
arms. In all cases this was done by analyzing the distribution of RFLP
alleles in the homozygous mutant class from an F2 population derived from
crosses between a full color stock (B, Pl, R-r) and the morphological
mutant.
| Mutant | Map position | Linked RFLP locus | Homozygous recessive class | Heterozy-gous class | Homozygous dominant class | % recomb. |
| ij2 | 1L | BNL7.25 | 8/9 | 1/9 | 0/9 | 6 |
| BNL8.29A | 9/9 | 0/9 | 0/9 | 0 | ||
| BNL6.32 | 7/9 | 2/9 | 0/9 | 11 | ||
| oy1 | 10S15 | PIO200075A | 20/31 | 9/31 | 2/31 | 21 |
| PIO060005 | 28/31 | 3/31 | 0/31 | 5 | ||
| py2 | 1L | BNL6.321 | 4/6 | 2/6 | 0/6 | 17 |
| sr2 | 10L98 | UMC44A | 16/18 | 2/18 | 0/18 | 6 |
| BNL10.13 | 17/20 | 2/20 | 1/20 | 10 | ||
| R1 | 17/20 | 3/20 | 0/20 | 8 | ||
| BNL17.02 | 17/20 | 3/20 | 0/20 | 8 | ||
| GLN1 | 10/18 | 7/18 | 1/18 | 25 | ||
| gl15 | 9L074 | BNL5.04 | 5/5 | 0/5 | 0/5 | 0 |
| BNL7.13 | 5/5 | 0/5 | 0/5 | 0 |
1Most distal probe on 1L; py2 was unlinked with BNL8.29A.
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