"Preferred
Set" of RFLP probes
--Jack Gardiner, Dave Hoisington,
Ed Coe, Shiaoman Chao and Susan Melia-Hancock
A set of 120 public-domain probes has been
selected, based upon clarity of definition of bands, demonstrated polymorphism,
and spacing on the linkage map. This Preferred Set will provide a baseline
series of probes that should aid and simplify characterization and mapping,
and is the series we intend to use in a core map (Hoisington and Coe, Stadler
Genetics Symposium, in press) against which defined-function probes, physical
points, and other traits are placed. The refined verification and standardization
of this set is now largely completed; probe distribution has been slowed
while this process was carried through.
As part of the characterization and
standardization of the probe set, we have generated a new F2 of Tx303 x
CO159 and will map the Preferred Set with this F2. The F2 plants were selfed,
and F3 pooled populations will be produced to derive an immortal F2.
Please Note: Notes submitted to the Maize Genetics Cooperation
Newsletter may be cited only with consent of the authors
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