The plant derived from the kernel exhibiting Ac activation was crossed as shown. From this cross 135 colorless
(no Ac) and 156 variegated (with
Ac) kernels were obtained. This is not significantly different than
the 1:1 ratio expected if a single Ac element is segregating. To
test the linkage of this Ac to the P locus plants from kernels
with and without Ac were grown to maturity at which point the ears
were scored for the presence of colored pericarp. 63 of 65 plants with
Ac carried the P-RR allele. In contrast only 1 of 29 plants
that did not receive Ac carried the P-RR allele. This yields
a recombination value between P-RR and Ac of 3.2 cM with
a standard error of 1.7. This tight linkage between the newly arisen P-RR
allele and the newly activated Ac element supports our original
hypothesis.
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