RALEIGH, NORTH CAROLINA
North Carolina State Univ. & USDA/ARS
Probes for the b-32 protein hybridize
to loci on 7L and 8L
--Hank W. Bass, Paul H. Sisco, David
L. Murray, and Rebecca S. Boston
The b-32 protein of maize endosperm is
encoded by a small family of genes (Hartings et al., MNL 63:29, 1989).
Expression of the b-32 genes has been reported to be affected by the zein
regulatory mutations opaque-2 and opaque-6 (= pro1) (Di Fonzo et
al., Mol. Gen. Genet. 212:481, 1988). We have used both cDNA and genomic
clones encoding b-32 to probe genomic Southern blots of Ben Burr's recombinant
inbred lines. Two loci hybridize to our b-32 probes, one on 8L between
BNLACT1 and BNL2.369 , and the other on 7L between BNL8.32
and BNL7.61. The cDNA probe, which corresponds to the major b-32
protein in kernels, shares greater identity with the locus on 8L than with
the locus on 7L.
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