In order to identify the maternal and/or
paternal origin of the ß-GLU activity, I have analyzed developing
kernels (from 3 DAP to maturity) of several reciprocal F1s between inbred
lines, characterized by the fast and slow variant of the enzyme. Had both
paternally and maternally inherited genes been expressed, I would have
expected to find the typical 3 band pattern, i.e. two homodimers and the
corresponding heterodimer. In each case only the maternally determined
isozyme was found instead, and the activity was strongest in the very early
stages of development (3-7 DAP) and progressively declined thereafter.
3 weeks after pollination (21 DAP) and later, no ß-GLU activity was
present either in whole kernels or in isolated tissues (scutellum, endosperm,
aleurone and pericarp). These preliminary data suggest that ß-GLU
activity in the developing kernel is not due to post zygotic expression
of the Glu1 genes, but rather inherited from the female parent.
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