--Peter A. Peterson
In a previous report (MNL:59) it was established that the mutability
of this allele is En-dependent. The phenotype of this allele (without
mutability) is a basic colored background that mutates to colorless sectors
(C to C-I). This would indicate that the insert suppresses
the C-I transcript (no suppression of anthocyanin color in C-I-m/C)
and subsequent excisions lead to release of that suppression followed by
restoration of the C-I effect (inhibition of color). That En
causes the sectoring is supported by the correlative effect of En
on wx-m8, a reporter allele for En. In the cross C x
C-I-m/C sh bz wx colored and colored-with-sectors kernels were tested.
In all cases, the colored selections were the C sh bz
chromosome and the sectored kernels were the C-I Sh Bz
chromosome. This is not absolute that it is En at the locus since
sectoring should always be accompanied by wx sectoring with the
wx-m8 allele. The exception (lack of wx sectoring) may be
due to change in wx-m8 to a non-responsive allele, which is a common
event.
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