--Scott Poethig
Wrk1 is a dominant, EMS-induced mutation that causes the endosperm to become deeply furrowed. Given its severe effect on endosperm development, it is interesting that this mutation has little or no seedling phenotype. Wrk1 was mapped to the short arm of chromosome 3 by M.G. Neuffer. Three point testcrosses involving Wrk1, Lg3, and Cg1 or Tp1 (Tables 1 and 2) demonstrate that Wrk1 is located 2.8 ± 0.9cM distal to Lg3. A two-point testcross between Wrk
Table 1. Lg3 + Cg1/+ Wrk1 + x +++
a) Lg3 + Cg1 | 96 |
+ Wrk1 + | 85 |
b) Lg3 + + | 25 |
+ Wrk1 Cg1 | 23 |
c) Lg3 Wrk1 + | 0 |
+ + Cg1 | 4 |
d) Lg3 Wrk1 Cg1 | 1 |
+ + + | 0 |
Total Progeny: | 234 |
% Recombination
Wrk1--Cg1: (b + d) = 20.9 ± 2.6 ~
Lg3--Wrk1: (c + d) = 2.1 ± 0.9 %
Lg3--Cg1: (b + c) = 22.2 ± 2.7 %
Table 2. Lg3 + Tp3/+ Wrk1 + x +++
a) Lg3 + Tp3 | 20 |
+ Wrk1 + | 41 |
b) Lg3 + + | 8 |
+ Wrk1 Tp3 | 14 |
c) Lg3 Wrk1 + | 1 |
+ + Tp3 | 3 |
Total Progeny: | 87 |
% Recombination
Wrk1--Tp3: (b) = 25.3 ± 4.7 %
Lg3--Wrk1: (c) = 4.6 ± 2.2 %
Lg3--Tp3: (b + c) = 29.9 ± 4.9%
and Cl1 indicated that these genes are 1.9 ± 0.5cM
apart (12/632). Only the non-wrinkled seeds were used in calculating this
latter recombination frequency because Cl could not be reliably
scored in Wrk1 seeds. Since cl1 is 5cM distal to Lg3,
these data suggest the following map order: Lg3--3--Wrk1--2--cl1.
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