The use of genetically blocked markers provides a powerful tool in analyzing the gene action sequence of the anthocyanin pathway. Homozygous recessive kernels of c1 and c2, 20-25 days after pollination, with exposed aleurone were used. These were placed directly on filter paper or 0.8% agar containing cinnamic acid, caffeic acid, naringenin and quercetin (1 mg/100 ml of dist. water). Anthocyanin was observed in about 25% of c1 kernels, fed with cinnamic acid and caffeic acid. In the case of c2, the kernels developed anthocyanin only with naringenin. The c2 kernels showed pigment on the crown, spreading gradually to the sides. In the case of c1 the crown which was in contact with the substrate showed very pale to almost no pigment, whereas lateral sides, which were directly exposed to light, developed pigment. Chromatographic and spectrophotometric studies of hydrolyzed samples suggest that the pigment is cyanidin.

Padma Balaravi and G. M. Reddy

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