A method for obtaining homozygous duplications

One of the problems with working with duplications is getting them homozygous. Duplications such as tDp3La and dDp3L-3S (MNL 46:138-142) and those produced by crossing translocations or inversions with overlapping break points are difficult to transmit through the pollen because of competition with normal pollen. A solution to this problem is to cross the duplication/normal heterozygotes with the appropriate TB-A translocation and then to self-fertilize the hypoploid progeny. The chromosome with the duplication is freed from competition with normal chromosomes and homozygous progeny results. This was attempted last summer. Because of poor growing conditions few hypoploids survived. It will be repeated.

G. G. Doyle

Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors.

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