4. A non‑liguleless allele of liguleless.
One F2 culture from a Bikini outcross segregated plants with the characteristic erect leaves of liguleless 1. Unlike lg1 the leaves of the new type possess a ligule and auricles. The sheath together with the ligule remain tightly wrapped as in the normal. But the midrib continues vertically upward instead of coming out at an angle from the top of the sheath. The seedlings look superficially like those of lg1 but the classification must be based upon the leaf angle only. Intercrosses with lg1 give seedlings typical of the new type. showing allelism. Since there is no weakening of the stem, the character has one advantage over lg1 but its comparative usefulness as a gene marker remains to be determined.
E. E. Dale and E. G. Anderson