7. Trisomic tests with unlinked genes. Trisomic tests for chromosome 6 and the following genes wore negative: zb, gl6, gl9 (trisomic plants had an excess of gl progeny as compared with the 2n), and v9. A seedling dwarf (one of Stadler's designated temporarily as ds-3) may be in chromosome 6 by this trisomic test.
Linkage data with other factors were obtained along with the tests for linkage in chromosome 6. The possible linkages are as follows:
Genes | N | Segregating for new characters |
χ2 for indep. test | Recom. �S.E. |
pr - ws | 104 | 3:1 | P = .02 | 35.5�6.1 |
Wc - si?* | 164 | 15:1 | P = .02 | |
Y - gl_11 | 283 | 3:1 | P = < .01 | 38.5�3.8 |
Y - w (in gl6 culture) | 202 | 3:1 | P = < .01 | 27.0�3.8 |
F1 - gls-3 | 276 | 3:1 | P = < .01 | 12.0�3.6 |
gls-3 - tw?** | 276 | excess for 15:1 | P = < .01 | 1.0�1.7 |
F1 -tw? | 276 | " | P = < .01 | |
1 aleur. factor - tw3 | 579 | " | P = .01 | 38.0�6.2 |
F1 - tw3 | 218 | " | P = .02-.01 | |
gl - tw3 | 561 | " | P = < .01 | 28.0�5.6 |
*This silky is one that appeared in an F2 of a single cross here.
**This tw was linked in coupling with the gls-3, one of Stadler's mutants.
Negative results by the χ2 for independence test were obtained for the following linkage tests: zb with lg; ws with Y Pl B rg; gls-1 with B lg nl?; nl? with B lg; ds-3 with su, pr Y colorless aleurone (9:7). gl6 with Y; gl9 with Y bt? and zg3 with Y and colorless aleurone (3:1).
C.R. Burnham and N. Klein