4. Trisomics - Seed weight. In order to get a relatively high frequency of trisomic plants the smaller seeds are often selected from a trisomic ear. A study was made to find how close a correlation exists between weight of seed and chromosome number and whether this correlation varies in different trisomic stocks.
Random samples of from 50 to 150 seeds were taken from trisomic ears. In some cases, however, only relatively small numbers of seeds were available. Each seed was weighed to the nearest .01 gram and placed in its weight class. In most cases the weights when plotted against number formed a unimodal curve In some, however, bimodal curves resulted (see III × lg2). The seeds were germinated in trays and roots taken before transplanting to the field. The results are expressed in table 1.
Table 1
Relative length of extra chromosome |
Trisomic Stock | Weight of seed in mg. |
% trisomics | No. of individuals |
% trisomics in random sample |
No. of individuals |
85 | II × L.F. Inbred | 140-210 | 82 | 38 | ||
220-230 | 53 | 59 | 50 | 139 | ||
240-260 | 19 | 42 | ||||
II × Inbred II | 160-220 | 14 | 7 | |||
230-240 | 56 | 16 | 37 | 43 | ||
250-280 | 30 | 20 | ||||
II × C. II Inbred | 130-200 | 87 | 30 | |||
210-230 | 49 | 35 | 52 | 93 | ||
230-260 | 18 | 28 | ||||
II × lg | 130-180 | 80 | 5 | |||
190-200 | 61 | 18 | 39 | 52 | ||
210-240 | 17 | 29 | ||||
79 | III × L.F. Inbred | 150-240 | 69 | 16 | 35 | 34 |
250-300 | 6 | 18 | ||||
III × Inbred II | 100-150 | 50 | 12 | |||
160-180 | 40 | 20 | 33 | 52 | ||
190-230 | 15 | 20 | ||||
III × lg 2 | 140-160 | 100 | 31 | |||
170-180 | 50 | 16 | 45 | 91 | ||
190-240 | 5 | 44 | ||||
78 | V × Inbred II | 120-160 | 65 | 52 | 52 | 89 |
32 | 37 | |||||
60 | VI × su2 | 140-200 | 77 | 30 | ||
210-220 | 12 | 42 | 30 | 103 | ||
230-260 | 10 | 31 | ||||
60 | VII × L.F. Inbred | 70-110 | 73 | 11 | 45 | 22 |
120-150 | 18 | 11 | ||||
VII × Inbred II | 70-120 | 63 | 16 | |||
130-140 | 39 | 23 | 40 | 58 | ||
150-200 | 21 | 19 | ||||
60 | VIII × L.F. Inbred | 110-170 | 63 | 38 | ||
170-180 | 44 | 43 | 32 | 146 | ||
190-220 | 6 | 65 | ||||
VIII × j | 200-230 | 33 | 18 | |||
240-260 | 33 | 18 | .27 | 45 | ||
270-320 | 0 | 9 | ||||
52 | IX × v wx | 120-160 | 46 | 13 | ||
170-180 | 71 | 24 | 22 | 113 | ||
190-220 | 3 | 76 | ||||
45 | X × L.F. Inbred | 220-250 | 48 | 54 | ||
260-270 | 12 | 52 | 26 | 149 | ||
280-310 | 12 | 43 | ||||
X × v18 | 200-230 | 58 | 12 | |||
240-250 | 35 | 20 | 37 | 49 | ||
260-270 | 24 | 17 |
Relative length of extra chromosome |
Trisomic stock |
Percent 2n + 1 plants in progeny |
No. of individuals |
Percent microspores with n + 1 chromosomes |
No. of individuals |
85 | II. x L.F. | 50 | 139 | 50 | 212 |
79 | III. x lg2 | 45 | 91 | 41 | 167 |
45 | X x L.F. | 26 | 149 | 34 | 190 |
X x v18 | 37 | 49 | 33 | 109 |
Relative length of extra chromosome |
Trisomic stock |
Percent microsporocytes with univalents in Met. I |
No. of individuals |
85 | II. x L.F. | 30 | 247 |
79 | III. x lg2 | - | - |
45 | X x L.F. | 49 | 372 |
X x v18 | 37 | 300 |