15. Linkage data for chrom. 10.

F1 genotype 0 1 2 1,2 Total
Rp  +   r 179 161 109 114 31 30 16 13 653
+   g1 + 340 223 61 29  
 
*   34.2% 9.3% 4.4% order is Rp-g1-r
 
Rp  +   + 223 90 40 13 366
+   li g1   24.5% 10.9% 3.6% order is Rp-li-g1

*(seedlings inoculated in flats and only resistant individuals transplanted to field, to prevent spreading the rust to other cultures).

Genes Phase XY Xy xY xy Total % recomb.
Rp D7 RS 409 127 133 41 710 50

Singleton reported 35% recombination between D7-G1 and 27% between D7-R. This suggests the order is G1-R-D7, but might be different. However, if D7 falls to the left of R it should show fairly strong linkage with Rp, but it does not. Therefore the order in chrom. 10 is:

Rp li g1 R d7
0 28 43 57 84

V. H. Rhoades