Maize Genetics Cooperation Newsletter vol 87 2013

 

As submitted by David Weber.

A reappraisal of Kindiger and HamannŐs 1993 protocol to produce large numbers of paternal haploids for cytoplasmic conversions

            Kindiger and Hamann (1993, Crop Sci. 33:342-344) developed a novel system to efficiently produce paternal (androgenic) haploids in maize.  They generated a maize tertiary trisomic plants with two normal chromosome 3s plus a B-3Ld chromosome in the inbred, W23.  The two normal chromosome 3s carried the ig1 allele and the dominant Ig1 allele was present on the B3-Ld chromosome.  The ig1 locus in chromosome 3 is close to the breakpoint in B-3Ld, and in the inbred, W23, ig1/ig1 plants are completely or almost completely male-sterile and plants with a dominant allele of the ig1 locus are male-fertile. 

They indicated that theoretically, transmission of the B-3Ld chromosome should be 50% through the female and only 2% through the pollen (Beckett 1983, Can. J Genet. Cytol. 25:346-353).  Therefore, when a 3(ig1)/3(ig1) female parent is crossed by a 3(ig1)/3(ig1)/B-3Ld(Ig1) male parent, nearly all of the progeny would be ig1/ig1 (and male-sterile in the inbred, W23) because the B-3Ld chromosome is rarely transmitted through the pollen.  Progeny that received the B-3Ld (Ig1) chromosome would be male-fertile and could easily be recognized.  Selfing of the male-fertile individuals 3(ig1)/3(ig1)/B-3Ld(Ig1) or crossing them to their male-sterile sibs 3(ig1)/3(ig1) can be used to maintain the 3(ig1)/3(ig1)/B-3Ld(Ig1) stock.

They also crossed 3(ig1)/3(ig1)/B-3Ld(Ig1) males by female parents with several different types of male-sterile cytoplasms and recovered 3(ig1)/3(ig1)/B-3Ld(Ig1) lines with the male-sterile cytoplasms.

Seed of selfed 3(ig1)/3(ig1)/B-3Ld(Ig1) plants and seed of 3(ig1)/3(ig1) plants with L, MY, ME, S, SD, VG, CA, C, and Q male-sterile cytoplasms crossed as female parents by 3(ig1)/3(ig1)/B-3Ld(Ig1) male parents were obtained from the Maize Genetics Stock Center, Univ. of IL and planted in field plantings in 1998.  These stocks were originally provided to the Maize Genetics Stock Center by KindigerŐs lab.  A high frequency of distinctively smaller plants were observed in field-plantings of each of these stocks as shown in the following table:

Cross                                                                  cytoplasm    # grew     # small    % small

3-ig1/3-ig1/B-3Ld-Ig1 self                                     N               111           22            19.8

3-ig1/3-ig1 x 3-ig1/3-ig1/B-3Ld-Ig1                     L                15             3              21.3

3-ig1/3-ig1 x 3-ig1/3-ig1/B-3Ld-Ig1                     MY            17             5              30.7

3-ig1/3-ig1 x 3-ig1/3-ig1/B-3Ld-Ig1                     ME            15             2              13.3

3-ig1/3-ig1 x 3-ig1/3-ig1/B-3Ld-Ig1                     S                79             3              3.7

3-ig1/3-ig1 x 3-ig1/3-ig1/B-3Ld-Ig1                     SD             19             8              29.6

3-ig1/3-ig1 x 3-ig1/3-ig1/B-3Ld-Ig1                     VG             26             2              7.7

3-ig1/3-ig1 x 3-ig1/3-ig1/B-3Ld-Ig1                     CA             22             6              21.4

3-ig1/3-ig1 x 3-ig1/3-ig1/B-3Ld-Ig1                     C                120           22            14.0

3-ig1/3-ig1 x 3-ig1/3-ig1/B-3Ld-Ig1                     Q               126           53            33.3

Total                                                                                            550           126         22.9

 

The smaller plants definitely did not have the morphology of a haploid plant; instead, they had a morphology that was typical of a monosomic-3 plant or a plant that was hypoploid for the long arm of chromosome 3.  Monosomic-3 plants and plants hypoploid for 3L have a similar morphology.  They are much smaller and have more upright leaves that are thicker, shinier, and narrower than those in their diploid siblings.  Chromosome counts were carried out on several of the smaller plants, and they each had 20 chromosomes.

Therefore, the smaller plants produced by this system are not haploids; however, they appear to be hypoploids for the long arm of chromosome 3, and therefore appear to have one normal chromosome 3 and a 3B chromosome.  Such plants would be expected if the complete B-3Ld translocation was present in the male parent.

The Maize Genetics Stock Center (personal communication) confirms that the Kindiger ig1 maintainer stock carries the complete TB-3Ld translocation—crosses of the maintainer stock to a1 testers segregated for colorless kernels with colored plumules.

 

Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of authors.