Maize Genetics Cooperation
Newsletter vol 85 2011
Linkage tests of waxy1 marked T3-9 reciprocal
translocations.
‑‑Jackson, JD
‑‑Stinard, PS
In the collection of A-A translocation stocks maintained at MGCSC is a series of waxy1-marked translocations that are used for mapping unplaced mutants. Over the last decade, new wx1-marked translocations have been introduced into this series and are in a conversion program to convert each translocation to the inbred backgrounds M14 and W23. These inbred conversions are then crossed together to produce vigorous F1's to fill seed requests. Below is a summary of the linkage results for some of these stocks. Additional translocation stocks will be tested as time allows.
We report here the positive results of two- and three-point linkage tests for two of these translocations: wx1 T3-9(034-11) (Tables 1-3) and wx1 T3-9g (Tables 4 and 5). The linkage tests were set up as modified backcrosses as indicated. A Findley source of wx1 T3-9g showed no linkage with its appropriate markers and was discarded.
The data reported here were compiled by Janet Day Jackson before her sudden and untimely death.
Table 1. wx1 T3-9(034-11) (3L.46; 9S.36), E. G. Anderson source #1.
Two-point linkage data for gl6 - wx1 T3-9(034-11).
Testcross: gl6 wx1 X [Gl6 wx1 T3-9(034-11) X gl6 Wx1 N]
|
Region |
Phenotype |
No. |
Totals |
|
0 |
Wx gl |
391 |
|
|
|
wx Gl |
471 |
862 |
|
1 |
Wx Gl |
76 |
|
|
|
wx gl |
90 |
166 |
|
|
|
|
|
|
Total |
|
|
1028 |
% recombination gl6 – wx1 = 16.1 +/- 1.1%
Table 2. wx1 T3-9(034-11) (3L.46; 9S.36), E. G. Anderson source #1.
Three-point linkage data for gl6 – lg2 - wx1 T3-9(034-11).
Testcross: [Gl6 Lg2 wx1 T3-9(034-11) X gl6 lg2 Wx1 N] X gl6 lg2 wx1 N
|
Region |
Phenotype |
No. |
Totals |
|
0 |
gl lg Wx |
183 |
|
|
|
Gl Lg wx |
189 |
372 |
|
1 |
gl Lg wx |
71 |
|
|
|
Gl lg Wx |
68 |
139 |
|
2 |
Gl Lg Wx |
24 |
|
|
|
gl lg wx |
32 |
56 |
|
1 + 2 |
gl Lg Wx |
3 |
|
|
|
Gl lg wx |
16 |
19 |
|
|
|
|
|
|
Total |
|
|
586 |
% recombination gl6 – lg2 = 27.0 +/- 1.8
% recombination lg2 – wx1 = 12.8 +/- 1.4
% recombination gl6 – wx1 = 39.8 +/- 2.0
Table 3. wx1 T3-9(034-11) (3L.46; 9S.36), E. G. Anderson source #2.
Note: This source of wx1 T3-9(034-11) was maintained independently of the source tested in Tables 1 and 2 due to a pedigree error labeling it as T8-9(034-11).
Three-point linkage data for gl6 – lg2 - wx1 T3-9(034-11).
Testcross: [Gl6 Lg2 wx1 T3-9(034-11) X gl6 lg2 Wx1 N] X gl6 lg2 wx1 N
|
Region |
Phenotype |
No. |
Totals |
|
0 |
gl lg Wx |
557 |
|
|
|
Gl Lg wx |
580 |
1137 |
|
1 |
gl Lg wx |
73 |
|
|
|
Gl lg Wx |
63 |
136 |
|
2 |
Gl Lg Wx |
48 |
|
|
|
gl lg wx |
53 |
101 |
|
1 + 2 |
gl Lg Wx |
0 |
|
|
|
Gl lg wx |
10 |
10 |
|
|
|
|
|
|
Total |
|
|
1384 |
% recombination gl6 – lg2 = 10.5 +/- 0.8
% recombination lg2 – wx1 = 8.0 +/- 0.7
% recombination gl6 – wx1 = 18.6 +/- 1.0
Table 4. wx1 T3-9g(F-24) (3L.40; 9L.14), Robertson source.
Three-point point linkage data for gl6 - lg2 - wx1 T3-9g.
Testcross: [Gl6 Lg2 wx1 T3-9g X gl6 lg2 Wx1 N] X gl6 lg2 wx1 N
|
Region |
Phenotype |
No. |
Totals |
|
0 |
gl lg Wx |
69 |
|
|
|
Gl Lg wx |
49 |
118 |
|
1 |
gl Lg wx |
4 |
|
|
|
Gl lg Wx |
2 |
6 |
|
2 |
Gl Lg Wx |
13 |
|
|
|
gl lg wx |
2 |
15 |
|
1 + 2 |
gl Lg Wx |
0 |
|
|
|
Gl lg wx |
3 |
3 |
|
|
|
|
|
|
Total |
|
|
142 |
% recombination gl6 – lg2 = 6.3 +/- 2.0
% recombination lg2 – wx1 = 12.7 +/- 2.8
% recombination gl6 – wx1 = 19.0 +/- 3.3
Table 5. wx1 T3-9g(F-24) (3L.40; 9L.14), Robertson source.
Two-point linkage data for lg2 - wx1 T3-9g.
Testcross: [Lg2 wx1 T3-9g X lg2 Wx1 N] X lg2 wx1 N
|
Region |
Phenotype |
No. |
Totals |
|
0 |
Wx lg |
400 |
|
|
|
wx Lg |
394 |
794 |
|
1 |
Wx Lg |
56 |
|
|
|
wx lg |
17 |
73 |
|
|
|
|
|
|
Total |
|
|
867 |
% recombination lg2 – wx1 = 8.4 +/- 0.9
Please Note: Notes submitted to the Maize Genetics Cooperation
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