Maize Genetics Cooperation Newsletter vol 84 2010

Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of authors.

Linkage Tests of waxy1 Marked Reciprocal Translocations obtained from the collection of William Findley

Janet Day Jackson and Philip Stinard

Maize Genetics Cooperation � Stock Center

Urbana, IL

In the collection of A-A translocation stocks maintained at MGCSC is a series of waxy1-linked translocations that are used for mapping unplaced mutants. Over the last decade, new wx1-linked translocations have been introduced into this series from various Cooperators.

We report here the positive results of two-point linkage tests for three of these new accessions. The linkage tests were set up as modified backcrosses as indicated. These new wx1 marked translocations have been propagated and are now available for distribution. Additional Findley translocation stocks will be tested as time allows.

Table 1. wx1 T4-9e (4S.53; 9L.26)

A) The Findley source showed linkage of wx1 with su1.

Two-point linkage data for su1-wx1 T4-9e

Testcross: [Su1 wx1 T4-9e x su1 Wx1 N] x su1 wx1 N

source:2004-1216-3^Findley

Region	Phenotype	No.	Totals
0	+ wx	542
	su Wx	475	1017
1	+ Wx	146
	su wx	46	192 / 1209

% recombination su1-wx1= 15.9 + 1.1

Table 2. wx1 T4-9g (4S.27; 9L.27)

A) The Findley source showed linkage of wx1 with su1.

Two-point linkage data for su1-wx1 T4-9g

Testcross: [Su1 wx1 T4-9g x su1 Wx1 N] x su1 wx1 N

source:2004-1217-3^Findley

Region	Phenotype	No.	Totals
0	+ wx	393
	su Wx	328	721
1	+ Wx	128
	su wx	90	218 / 939

% recombination su1-wx1= 23.2 + 1.4

Table 3. wx1 T7-9(027-9) (7L.61; 9S.18)

The Findley Source showed linkage of wx1 with gl1.

Two-point linkage data for gl1-wx1 T7-9(027-9).

Testcross: [Gl1 wx1 T7-9(027-9) x gl1 Wx1 N] x gl1 wx1 N

source:2004-1223-1^Findley

Region	Phenotype	No.	Totals
0	Wx gl	633
	wx +	710	1343
1	Wx +	144
	wx gl	122	266/1609

% recombination gl1-wx1= 16.5+ 0.9