Maize Genetics Cooperation Newsletter vol 84 2010

Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of authors.

 

 

 

Linkage Tests of waxy1 Marked Reciprocal Translocations obtained from the collection of William Findley

 

                                                      Janet Day Jackson and Philip Stinard

                                                      Maize Genetics Cooperation Stock Center

                                                      Urbana, IL

 

In the collection of A-A translocation stocks maintained at MGCSC is a series of waxy1-linked translocations that are used for mapping unplaced mutants.  Over the last decade, new wx1-linked translocations have been introduced into this series from various Cooperators.

We report here the positive results of two-point linkage tests for three of these new accessions. The linkage tests were set up as modified backcrosses as indicated.  These new wx1 marked translocations have been propagated and are now available for distribution. Additional Findley translocation stocks will be tested as time allows.

 

Table 1. wx1 T4-9e (4S.53; 9L.26)

 

A) The Findley source showed linkage of wx1 with su1.

 

Two-point linkage data for su1-wx1 T4-9e

Testcross:   [Su1 wx1 T4-9e x su1 Wx1 N] x su1 wx1 N

 

source:2004-1216-3^Findley

Region

Phenotype

No.

Totals

0

+ wx

542

 

 

su Wx

475

1017

1

+ Wx

146

 

 

su wx

46

192 / 1209

% recombination su1-wx1= 15.9 + 1.1

 

 

Table 2. wx1 T4-9g (4S.27; 9L.27)

 

A) The Findley source showed linkage of wx1 with su1.

 

Two-point linkage data for su1-wx1 T4-9g

Testcross:   [Su1 wx1 T4-9g x su1 Wx1 N] x su1 wx1 N

 

source:2004-1217-3^Findley

Region

Phenotype

No.

Totals

0

+ wx

393

 

 

su Wx

328

721

1

+ Wx

128

 

 

su wx

90

218 / 939

% recombination su1-wx1= 23.2 + 1.4

 

 

Table 3. wx1 T7-9(027-9) (7L.61; 9S.18)

 

The Findley Source showed linkage of wx1 with gl1.

 

Two-point linkage data for gl1-wx1 T7-9(027-9).

Testcross:   [Gl1 wx1 T7-9(027-9) x gl1 Wx1 N] x  gl1 wx1 N

 

source:2004-1223-1^Findley

Region

Phenotype

No.

Totals

0

Wx gl

633

 

 

wx +

710

1343

1

Wx +

144

 

 

wx gl

122

266/1609

% recombination gl1-wx1= 16.5+ 0.9