Maize Genetics Cooperation Newsletter vol 84 2010

Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of authors.

 

 

Linkage Tests of waxy1 Marked Reciprocal Translocations obtained from the collection of Don Robertson

 

                                                      Janet Day Jackson and Philip Stinard

                                                      Maize Genetics Cooperation � Stock Center

                                                      Urbana, IL

 

In the collection of A-A translocation stocks maintained at MGCSC is a series of waxy1-linked translocations that are used for mapping unplaced mutants.  Over the last decade, new wx1-linked translocations have been introduced into this series and are in a conversion program to convert each translocation to the inbred backgrounds M14 and W23.  These inbred conversions are then crossed together to produce vigorous F1's to fill seed requests.  Below is a summary of the linkage results for some of these stocks.  Additional translocation stocks will be tested as time allows. 

We report here the positive results of two-point linkage tests with f1 and P-ww for 6 of these new accessions: for f1-wx1 T1-9�s (Tables 1& 2) and for P1-ww-wx1 T1-9�s (Tables 3-6).  The linkage tests were set up as modified backcrosses as indicated.  These new wx1 marked translocations have been converted and F1�s are now available for distribution.  A sixth translocation was found to show no linkage with its appropriate marker stock.

 

 

Table 1. wx1 T1-9(8886) (1L.33; 9L.23)

wx29E

A) The Robertson source showed linkage of wx1 with f1.

 

Two-point linkage data for f1-wx1 T1-9(8886)

Testcross:   [F1 wx1 T1-9(8886) x f1 Wx1 N] x f1 wx1 N

 

source: Robertson 86-2247-3

Region

Phenotype

No.

Totals

0

Wx f

98

 

 

wx +

91

189

1

Wx+

10

 

 

wx f

10

20 / 209

% recombination f1-wx1= 9.6+ 2.0

 

 

Table 2. wx1 T1-9(4398) (1L.51; 9S.19)

wx29F

 

A) The Robertson source showed linkage of wx1 with f1.

 

Two-point linkage data for f1-wx1 T1-9(4398)

Testcross:   [F1 wx1 T1-9(4398) x f1 Wx1 N] x f1 wx1 N

 

source: Robertson 67-5242-10

Region

Phenotype

No.

Totals

0

Wx f

535

 

 

wx +

582

1117

1

Wx+

29

 

 

wx f

26

55 / 1172

% recombination f1-wx1= 4.7+ 0.6

 

 

Table 3. wx1 T1-9(8460) (1S.13; 9L.24)

wx29A

 

Two-point linkage data for P1-ww-wx1 T1-9(8460)

Testcross:   [P1-ww Wx1 N x P1-wr wx1 T1-9(8460)] x P1-ww wx1 N

 

source: Robertson 68-7246-4

Region

Phenotype

No.

Totals

0

P1-wr wx

122

 

 

P1-ww Wx

115

237

1

P1-ww wx

39

 

 

P1-wr Wx

47

86 / 323

% recombination P1-ww-wx1=26.6+2.5

 

 

Table 4. wx1 T1-9(8919) (1S.21; 9L.20)

wx29B

 

Two-point linkage data for P1-ww-wx1 T1-9(8919)

Testcross:   [P1-ww Wx1 N x P1-wr wx1 T1-9(8919)] x P1-ww wx1 N

 

source: Robertson 89-3002-6

Region

Phenotype

No.

Totals

0

P1-wr wx

83

 

 

P1-ww Wx

75

158

1

P1-ww wx

16

 

 

P1-wr Wx

20

36 / 194

% recombination P1-ww-wx1=18.6+2.8

 

 

 

Table 5. wx1 T1-9(8129) (1S.53; 9L.27)

wx29C

 

Two-point linkage data for P1-ww-wx1 T1-9(8129)

Testcross:   [P1-ww Wx1 N x P1-wr wx1 T1-9(8129)] x P1-ww wx1 N

 

source: Robertson 67-5354-4

Region

Phenotype

No.

Totals

0

P1-wr wx

315

 

 

P1-ww Wx

288

603

1

P1-ww wx

13

 

 

P1-wr Wx

66

79 / 682

% recombination P1-ww-wx1=11.6+1.2

 

 

 

Table 6. wx1 T1-9(024-7) (1S.71; 9L.13)

wx29D

 

Two-point linkage data for P1-ww-wx1 T1-9(024-7)

Testcross:   [P1-ww Wx1 N x P1-wr wx1 T1-9(024-7)] x P1-ww wx1 N

 

source: Robertson 68-7256-1

Region

Phenotype

No.

Totals

0

P1-wr wx

276

 

 

P1-ww Wx

265

541

1

P1-ww wx

35

 

 

P1-wr Wx

42

77 / 618

% recombination P1-ww-wx1=12.5+1.3

 

 

 

wx1 T8-9(4643) (8S.37; 9L.11)

 

A) The Robertson sources showed no linkage of wx1 with v16.