Maize Genetics Cooperation Newsletter vol 81 2007

 

 

HONOLULU, HAWAII

University of Hawaii

 

Near isogenic lines (NIL) of inbred Hi27

--Brewbaker, JL; Josue, AD

 

          The Hi27 NIL series was initiated in 1967 to provide tropically-adapted mutants to scientists working in the tropics (MNL 42:37-38).  Each mutant was to be backcrossed at least six times to  Hi27, a hardy tropical flint inbred that we selfed out of inbred CM104, created in India as a sib-line from the Colombian flint Amarillo Theobromina (pedigree = A Theo 21-B-6#-15-7#).  Hi27 generally tolerates most tropical diseases and environmental insults, and is homozygous for loci such as A1 A2 b Bz C-I Mv p-ww Pl pr r Y.  In 1995, we published a list of mutant loci that had been entered through backcrossing (MNL 69:58-59).  All of the mutants listed at that time have now been backcrossed six or more times to Hi27, with the following changes:

          (1) Mutants that could not be maintained from the 1995 list:  bk, bt2, lc, mn, pg2, rf, rt, v2 and w3

          (2) Mutants that have been added to this list:  a2, bk2, bt1-A, c2, j2, ms6, ms8, o5, Tlr, y8 and y11

          (3) New mutants (temporary symbols) under study:  blo (blotch), bst (brown-stripe), dcb (double-cob), lc2 (leaf-color), lfl (leaf-fleck), nl3 (narrow-leaf), os (opaque-small), sky (skinny) and zb232 (zebra)

          The complete NIL set now includes 97 mapped genes and the 9 mutants under study.  More than 200 genotypes are now available, including many digenic and multi-genic combinations such as bm3 gt and C sh bz wx.  All are being provided to the Maize Genetics Coop, e.g., symbolized sh2^Hi27.

 

 

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