Designation of Les21
--Neuffer, MG; England, D

Plants of Les*-1442 are yellowish green and grow slowly because of the many small chlorotic patches that appear below the leaf surface. Also fewer tiny round white necrotic lesions appear to arise from the chlorotic patches as the leaves mature. The necrotic lesions may enlarge and coalesce at senescence. Significant waxy translocation linkage data show no linkage to 22 of 23 translocation sites, covering the major portions of all 20 chromosome arms. This indicates that this mutant may be located in one of the regions not covered by these markers; namely, the distal end of 4S, 5S, 6L, 7S, 8L or 9L. The one missing test was for 7S. Since all the other designated lesion mutants have known locations, we have concluded that Les*-1442 could not be allelic to any of those; but an additional lesion site, which is designated Les21. The data are shown below:

Les*-1442:
 
Marker Breakpoint # Plts M Wx N Wx M wx N wx r ± SE chi sq
T1-9c S.48L.22 198 53 46 49 50 48. 0±3.5 .32
T1-9(4995) L.19S.20 136 33 36 38 29 45. 6±4.3 1.08
T1-9(8389) L.74L.13 66 21 13 18 14 47. 0±6.1 .21
T2-9b S.18L.22 151 30 48 30 43 48. 3±4.1 .11
T2-9c S.49L.33 177 37 45 34 61 44. 6±3.7 1.60
T2-9d L.83L.27 201 69 61 36 35 48. 3±3.5 .10
T3-9(8447) S.44L.14 136 29 28 36 43 47. 1±4.3 .37
T3-9c L.09L.12 100 18 29 23 30 48. 0±5.0 .27
T3-9(8562) L.65L.22 81 22 18 19 22 45. 7±5.5 .61
T4-9g S.27L.27 124 32 32 29 31 49. 2±4.5 .03
T4-9(5657) L.33S.25 82 21 22 20 19 48. 8±5.5 .05
T4-9b L.90L.29 270 69 69 65 67 49. 6±3.0 .02
T5-9c S.07L.10 114 29 30 26 29 49. 1±4.7 .04
T5-9a L.69S.17 211 67 76 34 34 47. 9±3.4 .18
T6-9a S.79L.40 154 38 39 34 43 47. 4±4.0 .42
T6-9b L.10S.37 78 15 24 19 20 44. 9±5.6 .83
T7-9(4363) .00.00 150 23 52 32 43 44. 0±4.1 2.33
T7-9a L.63S.07 148 16 65 16 51 45. 3±4.1 .37
T8-9d L.09S.16 91 18 32 18 23 45. 1±5.2 .59
T8-9(6673) L.35S.31 121 35 41 23 22 47. 1±4.5 .29
T9-10b S.13S.40 255 53 75 57 70 48. 2±3.1 .31
T9-10 (8630) S.28L.37 243 53 70 66 54 44. 0±3.2 3.45

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