Mature and immature (16-18 days after pollination) embryos of seven inbreds were tested for callusing ability with and without silver nitrate (10 mg/l). MS medium was supplemented with 2,4-D (2 mg/l), casein hydrolysate (1 g/l), inositol (100 mg/l), sucrose (3%) and agar (0.7%). Fifty mature and fifty immature embryos from each inbred were cultured in the media with silver nitrate, and the same number of embryos were cultured without silver nitrate. Callus induction frequency (%) was taken 20 days after inoculation. For all the inbreds, better callus induction was observed with silver nitrate either in mature or immature embryos (Table 1). It was also noted that immature embryo was better than mature embryo to induce callus for all the inbreds. The variation in callus induction frequency was significant among the inbreds, between two different embryo ages and also between the silver nitrate treatments.
Table 1. Impact of silver nitrate and embryo age on callusing ability
of different inbreds.
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| 2100 | 47.02 | 9.23 | 62.19 | 20.40 |
| 2702 | 38.92 | 10.20 | 58.62 | 15.23 |
| 2122 | 65.02 | 25.90 | 81.23 | 39.42 |
| 3498 | 72.91 | 30.00 | 80.21 | 35.40 |
| 3508 | 80.29 | 40.21 | 85.20 | 44.21 |
| 3994 | 35.80 | 6.29 | 50.29 | 16.20 |
| 4603 | 50..02 | 20.41 | 90.49 | 40.21 |
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