Anther culture of tetraploid maize

In the last year over 1000 anthers of tetraploid maize were inoculated on Zheng-14 medium. The other procedures followed were the same as reported by Ting et al. (1981). It was observed that over two percent of the anthers responded positively by growing into callus or embryoid. Some of the embryoids regenerated into plantlets. Hence, it is possible to dissect tetraploid maize into diploids by anther culture in vitro. Whether the regenerants are mostly diploid is currently under investigation.

Sandha Raja and Y. C. Ting

Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors.

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